SeqDesk · original analysis
Citation adoption for curated taxonomic profiling & classification tools, counted weekly from OpenAlex.
Software health, not citations: GitHub/GitLab stars and last-commit date for each tool’s canonical source repo, as a point-in-time snapshot (2026-06-30). Bars in the last-commit chart show how recently each repo was touched — active, stale, archived or gone. Star bars are tinted to match each tool’s citation line above.
Use cumulative view for long-run adoption and annual view for citation momentum; log scale makes smaller tools readable without changing the counts. Counts are annual OpenAlex cited-by tallies and reflect all uses of each paper, not only use inside the selected workflow; broad tools can therefore look larger than workflow-specific methods. The still-filling current year (2026) is omitted from the lines.
Each dot is one of 22 curated tools with a datable code repository (0 web-only / SourceForge tools without a public commit date aren't shown). Higher means more cited in 2025, the latest complete year (log scale); further left means more recently updated (square-root scale). Filled dots are actively maintained — a commit within the last 18 months. The upper-left gathers the field's well-kept, widely-used staples: fastp, SAMtools, DADA2, SPAdes, Prokka, DIAMOND and more, updated regularly and cited thousands of times a year. Maintenance and citations are only loosely linked (Spearman ρ ≈ −0.46): a citation counts every paper that mentions a method, so a quieter commit history does not mean a tool is unused or unmaintained — many stable, widely-relied-on tools rarely need new commits, and development sometimes moves to mirrors this snapshot can't see. Source: OpenAlex citation graph + GitHub/GitLab activity, as of 2026-06-25.
The leading taxonomic profiling & classification tool is recomputed for each complete calendar year by cumulative citations — a 3-way hand-off across 14 years.
cites: grouped by year when available; counts_by_year fallback when the grouped endpoint is budget-limited)scripts/check-tool-trends.mjs| Tool | Group | Lifetime citations | Peak year | Source | Stars | Last commit | Status |
|---|---|---|---|---|---|---|---|
| DIAMOND | Taxonomic profiling & classification | 15,142 | 2025 (2.2K/yr) | GitHub ↗ | 1.3K | this month | active |
| Kraken 2 | Taxonomic profiling & classification | 7,128 | 2025 (1.6K/yr) | GitHub ↗ | 916 | this month | active |
| GTDB-Tk | Taxonomic profiling & classification | 5,465 | 2024 (1.0K/yr) | GitHub ↗ | 609 | 1 mo ago | active |
| Kraken | Taxonomic profiling & classification | 4,875 | 2020 (601/yr) | GitHub ↗ | 226 | 4.8 yr ago | stale |
| MetaPhlAn2 | Taxonomic profiling & classification | 2,424 | 2021 (369/yr) | GitHub ↗ | 413 | this month | active |
| Bracken | Taxonomic profiling & classification | 2,298 | 2025 (506/yr) | GitHub ↗ | 354 | 4 mo ago | active |
| Kaiju | Taxonomic profiling & classification | 2,270 | 2024 (322/yr) | GitHub ↗ | 303 | this month | active |
| MetaPhlAn | Taxonomic profiling & classification | 1,902 | 2021 (218/yr) | GitHub ↗ | 413 | this month | active |
| MEGAN CE | Taxonomic profiling & classification | 1,849 | 2021 (250/yr) | GitHub ↗ | 75 | 2.0 yr ago | stale |
| Centrifuge | Taxonomic profiling & classification | 1,532 | 2024 (202/yr) | GitHub ↗ | 275 | 3 mo ago | active |
| MetaPhlAn 4 | Taxonomic profiling & classification | 1,347 | 2025 (576/yr) | GitHub ↗ | 413 | this month | active |
| PhyloSift | Taxonomic profiling & classification | 716 | 2020 (98/yr) | GitHub ↗ | 82 | 10 yr ago | stale |
| CLARK | Taxonomic profiling & classification | 693 | 2019 (87/yr) | GitHub ↗ | 2 | this month | active |
| CAT/BAT | Taxonomic profiling & classification | 592 | 2024 (107/yr) | GitHub ↗ | 231 | 3 mo ago | active |
| KrakenUniq | Taxonomic profiling & classification | 525 | 2025 (91/yr) | GitHub ↗ | 252 | 2.4 yr ago | stale |
| mOTUs2 | Taxonomic profiling & classification | 495 | 2022 (102/yr) | GitHub ↗ | 177 | this month | active |
| sourmash | Taxonomic profiling & classification | 365 | 2025 (60/yr) | GitHub ↗ | 548 | this month | active |
| MMseqs2 taxonomy | Taxonomic profiling & classification | 313 | 2025 (77/yr) | GitHub ↗ | 2.1K | this month | active |
| sylph | Taxonomic profiling & classification | 90 | 2025 (47/yr) | GitHub ↗ | 312 | 5 mo ago | active |
| ganon | Taxonomic profiling & classification | 88 | 2022 (18/yr) | GitHub ↗ | 106 | this month | active |
| Metabuli | Taxonomic profiling & classification | 34 | 2025 (23/yr) | GitHub ↗ | 179 | 3 mo ago | active |
| Centrifuger | Taxonomic profiling & classification | 32 | 2025 (18/yr) | GitHub ↗ | 92 | this month | active |
A curated cohort of taxonomic profiling & classification tools, each tracked by the full citation history of its method paper — re-counted weekly straight from the OpenAlex citation graph. Only the published method papers and their public citation counts are used. · show all tool groups · back to all research data